On-demand immobilization of
Slice format allows the use of tools, equipment, and software for conventional DNA or protein microarrays
CUSTOM DEVELOPED MICROARRAYS
Different cells, tissues and membrane domains can be immobilized
Radioligand, enzymatic assays, mass spectrometry, and immunoassays can be done in CMMAs.
The functionality of proteins is maintained during the whole immobilization process.
Simultaneous analysis of different tissues, cells, and replicates. Reducing the amount of time.
Radioligand binding assays
Microarrays are incubated with a specific radioligand and after washing and drying process, are exposed to a radiosensitive film, along with a commercial standard that allows for subsequent calibration. The film is quantified and analyzed employing image analysis software.
Enzymatic activity assays
Microarrays are incubated with a specific substrate of the enzyme under study and the modulating molecule. After a washing step, the image (colorimetry, fluorescence ...) is scanned, quantified, and analyzed. Microarray format allows studying in a single experiment different samples and tissues simultaneously, enabling the determination of dose-effect curves and pharmacological parameters.
Functional coupling assays
Microarrays are incubated with the specific ligand of a G protein-coupled receptor and [35S]GTPγS. The ligand activates the receptor and the G protein that exchanges GDP with the nonhydrolyzable analog of GTP, [35S]GTPγS. The microarray is exposed to a radiosensitive film with a [14C] standard that allows to calibrate results. After being developed is quantified and analyzed employing image analysis software.
Microarrays developed by IMG Pharma can also be employed in immunoassays, as both slides and microfluidics chips used to show low autofluorescence. The inclusion of calibration points allows the quantification of obtained images. Various types of immunoassays can be carried out on IMG Pharma microarrays: direct, indirect, applying methods of signal amplification, etc.
As our printing technology preserves the lipidic environment of cell membranes, is possible to employ MALDI-MS to determine lipidic profiles of immobilized samples, obtaining detailed lipidic profiling of samples (usually hundreds of lipids in each sample). In addition:
Avoids time-consuming separation, extraction, or purification steps.
Labelling free: no need of immunostaining, neither of radiometric technics (no radioligands needed).
Proprietary software for rapid alignment, normalization and statistical data analysis.